A sustainable shift in ULT freezer temperatures at the UCL Institute of Prion Diseases
By e.wylde, on 26 February 2025
In this blog, Professor Simon Mead and Azy Khalili talk about the sustainability takeaways from reducing freezer temperatures at the UCL Institute of Prion Diseases, from risk management to carbon and cost savings. We also provide a snapshot of ultralow temperature (ULT) freezers across the Faculty.
ULT freezers are used for long-term storage of biomolecules, such as proteins and DNA. They typically provide a temperature between -40 to -80°C to protect samples from heat-associated denaturation. Historically, ULT freezers were set at -65°C or -70°C until manufacturers in the 80s and 90s marketed -80°C as the new standard. This was despite the lack of evidence showing a superior performance in terms of sample stability or recovery, not to mention the additional energy consumption and cost. After careful planning, in early 2024, staff at the UCL Institute of Prion Diseases (IOPD) made the decision to turn their ULT freezers down to -70°C. Now, a year later, they reflect on this decision in terms of sustainability benefits, sample integrity and cost savings.
Making the switch at the IOPD: Evaluating the potential risks
Before making the switch, careful planning and reporting was essential. Those at the IOPD needed to make sure that -70°C was acceptable to all before going ahead. This involved considering the potential risks.
One primary concern was sample integrity, particularly for long-term storage of sensitive biomaterials. However, existing studies indicated that many biological materials—including genomic DNA, fungal isolates, plasma antibodies, and cardiac biomarkers—remain stable at -70°C for extended periods. No compelling evidence supported the notion that -80°C is necessary for sample preservation over -70°C.
Another risk involved freezer performance during power outages. Lower storage temperatures theoretically provide a buffer against temperature fluctuations in case of failures. However, studies show that a freezer at -80°C only extends the time before reaching -20°C by 35 additional minutes compared to a freezer set at -70°C (19h 45m vs. 19h 10m). This difference was minimal, especially for modern, well-insulated freezers. Additionally, most research freezers at IOPD are connected to backup power and alarm systems, mitigating this risk.
Operationally, concerns may arise regarding staff and researcher acceptance of the change. A transparent communication strategy—including references to scientific literature and comparative data—helps ensure confidence in the switch. Regular audits of sample quality over time further reassures researchers that -70°C remains a reliable storage standard.
Cutting carbon and costs at IOPD
The transition from -80°C to -70°C at the IOPD presents significant sustainability benefits. The IOPD now have 34 freezers turned down to -70°C.
By lowering freezer temperatures to -70°C, IOPD has observed energy savings of approximately 32-33%, as confirmed by in-house measurements and external reports from manufacturers such as ThermoFisher and Eppendorf. This translates to lower electricity consumption, reducing institutional carbon footprints and operational costs.
At IOPD, these changes have led to an annual estimated cost saving of over £17,000 for 21 freezers and £5,800 for 12 Cryocube freezers, based on energy rates of 30p/kWh in London.
Furthermore, reducing freezer loads by discarding unnecessary or outdated samples enhances sustainability. Freezers with optimized space usage maintain stable internal temperatures, prolong equipment lifespan, and minimize maintenance issues. By implementing these changes, IOPD contributes to UCL’s broader sustainability goals, aligning with environmental responsibility and cost-efficiency in research operations.
Snapshot of ULT Freezer temperatures across the Faculty:
- UCL Queen Square Institute of Neurology: 94% of ULT Freezers are at -70°C. They hope to reach 100% soon
- UCL Division of Psychology and Language Sciences: 100% of ULT freezers are set to -70°C
- UCL Ear Institute: 100% (two out of two) ULT freezers both set to -70°C%
- UCL Division of Psychiatry: 67% (two out of three) freezers are set at -70°C. One remains at -80°C because this temperature was specified as the storage temperature in a clinical trial. Once the data for this trial has been analysed, the freezer will be set to -70°C
- UCL Institute of Ophthalmology: 29% (eight out of 28) freezers set at -70°C, with the rest at -80°C. They are taking steps to switch more freezers to a warmer temperature.